Why is my protein’s N-terminal blocked (and what can I do about it)?

Question:   I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation/sequencing?   Answer:   N-terminal sequencing...

Aug 31. 2017 | Ejvind Mørtz |
Preparation of sample for MALDI

What buffer to use for MALDI mass spectrometry analysis of a protein?

Question:   What buffer should I use for MALDI Mass Spectrometry analysis of my protein sample?   Answer:   In general, MALDI-MS is more tolerant to contamination...

Apr 28. 2016 | Ejvind Mørtz |

What does the Mascot Score mean?

Question:   I just got my protein identification report. What does the Mascot Score mean?   Answer:   The Protein Identification report contains a list of proteins that have...

Oct 9. 2015 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question:   How do I optimize electroblotting conditions onto PVDF membrane for N-terminal Edman sequencing? Our 12 kDa protein separates well on 1D...

Oct 13. 2013 | Ejvind Mørtz |

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

Aug 18. 2013 | Ejvind Mørtz |
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