Why is my protein’s N-terminal blocked (and what can I do about it)?

Question: Does a blocked N-terminal hinder Edman degradation? I found that the N-terminal of my protein is blocked. So, how can I...

Aug 31. 2017 | Ejvind Mørtz |

Buffers for MALDI mass spectrometry analysis of a protein

Question: What buffer should I use for MALDI analysis? What buffer should I use for MALDI Mass Spectrometry analysis of my protein...

Apr 28. 2016 | Ejvind Mørtz |

What does the Mascot Score mean?

Question: What does the Mascot Score mean? I just got my protein identification report. What does the Mascot Score mean? Answer: The Protein Identification...

Oct 9. 2015 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question: How do I optimize electroblotting conditions onto a PVDF membrane? Our 12 kDa protein separates well on 1D SDS PAGE but...

Oct 13. 2013 | Ejvind Mørtz |
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