2D PAGE preparation for MALDI ISD

1D SDS PAGE versus 2D PAGE. Any recommendations?

Question: Should I use 1D or 2D SDS PAGE? I’m interested in isolating and identifying cell surface receptor proteins. They typically have high...

Sep 18. 2016 | Ejvind Mørtz |

How much protein should I load for protein identification by mass spec

Question: How should I prepare my samples for 2D electrophoresis? How should I prepare my samples for 2D electrophoresis, and how much...

Jun 8. 2016 | Ejvind Mørtz |
Mass spectrometry analysis of protein

Common protein contaminants in mass spectrometric protein ID

Questions: Why was Keratin identified in my 1D gel band? Is the keratin determined by the protein identification service in my...

Jun 3. 2016 | Ejvind Mørtz |

What does the Mascot Score mean?

Question: What does the Mascot Score mean? I just got my protein identification report. What does the Mascot Score mean? Answer: The Protein Identification...

Oct 9. 2015 | Ejvind Mørtz |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

Optimize PVDF membrane electroblotting for N-terminal Edman sequencing

Question: How do I optimize electroblotting conditions onto a PVDF membrane? Our 12 kDa protein separates well on 1D SDS PAGE but...

Oct 13. 2013 | Ejvind Mørtz |

Host Cell Protein monitoring in process development – using mass spec

Mass spectrometry based Host Cell Protein identification and quantification The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...

Oct 2. 2009 | Ejvind Mørtz |
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