Why is my protein’s N-terminal blocked (and what can I do about it)?

Question:   I found that the N-terminal of my protein is blocked. How can I then analyze it by Edman degradation/sequencing?   Answer:   N-terminal sequencing...

Aug 31. 2017 | Ejvind Mørtz |
100% sequence coverage map of antibody

Antibody de-novo Sequencing: How to get 100% sequence coverage

Question:   Is it possible to perform a de-novo sequencing of my purified monoclonal antibody without the hybridoma cells?   Answer:   Yes! – Your purified antibody can...

Mar 17. 2017 | Fen Yang Reske-Nielsen |

Protocol for gel silver staining – mass spec & protein identification

Problems arising from using commercial staining kits: Scientists use silver staining for sensitive detection of proteins separated by 1D and 2D...

Aug 6. 2015 | Ejvind Mørtz |

N- and C-terminal protein sequencing using MALDI ISD

Structural characterization of purified natural and recombinant proteins traditionally includes N-terminal Edman sequencing to confirm 5-15 amino acid residues from...

Aug 18. 2013 | Ejvind Mørtz |
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