We offer two types of quantification services: Quantification based on amino acid analysis or based on mass spectrometry. The right service for you depends on your project.
Amino acid analysis:
Accurate amino acid analysis is based on the European Pharmacopoeia. Therefore, the service can be used to quantify the specific amount of purified proteins and peptides. It can also be used to determine the overall protein concentration in complex protein samples. The analysis includes an acidic hydrolysis of your protein sample, thus transforming all protein material into free amino acids.
- Quantification of protein or peptide >
First we hydrolyze your sample into free amino acids. Then we perform amino acid analysis and quantify the total protein content.
- Extinction coefficient determination >
We start with exact quantity of protein determined by amino acid analysis. Then we calculate the molar extinction coefficient from dilution series and the protein MW.
If you need to accurately quantify intact proteins or peptides you should use mass spectrometry.
- Quantification by MRM mass spectrometry >
Quantify specific proteins in very complex mixtures by Multiple Reaction Monitoring (MRM).
- Protein quantification by SWATH mass spectrometry >
Identify and quantify specific protein(s) using label free SWATH mass spectrometry.