Quantification

Amino acid analysis of free amino acids, peptides and proteins

How do you quantify your protein amount and determine the amino acid composition, with fast turnaround time and a low cost?

Or obtain the ID and quantity of process-related impurities, such as host cell proteins?

With Alphalyse quantification services, you do not need to setup a complicated and expensive assay at your own facility. By outsourcing...

How do you quantify your protein amount and determine the amino acid composition, with fast turnaround time and a low cost?

Or obtain the ID and quantity of process-related impurities, such as host cell proteins?

With Alphalyse quantification services, you do not need to setup a complicated and expensive assay at your own facility. By outsourcing this part of your product characterization, you have time and money on your hands to focus on development and moving through clinical phase trials.

We offer two types of quantitative amino acid analysis services: Quantification based on amino acid analysis or based on mass spectrometry. The right service for you depends on your project. Accurate quantification of amino acid composition is based on the European Pharmacopoeia.

Quantitative amino acid analysis

The amino acid analysis service can be used to quantify the specific amount of purified proteins and peptides. It can also be used to determine the overall protein concentration in complex protein samples. The quantitative analysis includes an acidic hydrolysis of your protein sample, thus transforming all protein material into free amino acids.

Quantification by mass spectrometry

Are you looking for an accurate quantification of intact proteins or peptides? Then you should use our analysis based on mass spectrometry.

Describe the problem you want us to solve, and we will design a service package that meets your needs.

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How do you perform Amino Acid quantification?

Amino acid analysis is the golden standard for accurate quantification of proteins. The amino acid analysis service is quite straightforward: First a hydrolysis of an accurate amount of sample using strong acid, and subsequently detection and quantification of individual amino acids.

Why perform Amino Acid Analysis?

Amino acid analysis can be used to determine the relative composition of amino acids in a protein. If the sequence is known you can use the analysis to find the absolute amount of a protein or peptide, or estimate the purity of a purified protein.

How do I determine the molar extinction coefficient of the protein in my buffer system?

You do this by A280 measurements of a dilution series of the protein in known concentrations or predicted by a theoretical calculation from the number of A280 absorbing residues.

Why should I use triplicate AAA?

It is a very robust analysis.
You can compare the results with the sequence.
It is linear over a broad concentration range.
Your data can be qualified, using internal and external reference standards.

Who is this for?

We primarily help customers within the pharmaceutical industry, and biotechs working on drug development, e.g.

Project managers

Working with process development and characterization projects.

Analytical experts

Protein scientists that work with ELISA, masss spectrometry, or CMC.