Quantification
Amino acid analysis of free amino acids, peptides and proteins
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Our experts in protein analysis by mass spectrometry can help you in the following areas:
We offer customized solutions, contact us to discuss your project.
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Set up meetingAmino acid analysis is the golden standard for accurate quantification of proteins. The amino acid analysis service is quite straightforward: First a hydrolysis of an accurate amount of sample using strong acid, and subsequently detection and quantification of individual amino acids.
Amino acid analysis can be used to determine the relative composition of amino acids in a protein. If the sequence is known you can use the analysis to find the absolute amount of a protein or peptide, or estimate the purity of a purified protein.
You do this by A280 measurements of a dilution series of the protein in known concentrations or predicted by a theoretical calculation from the number of A280 absorbing residues.
It is a very robust analysis.
You can compare the results with the sequence.
It is linear over a broad concentration range.
Your data can be qualified, using internal and external reference standards.
We primarily help customers within the pharmaceutical industry and biotechs working on drug development, e.g.
Working with process development projects.
Protein scientists that work with ELISA, masss spectrometry, or CMC.