You can use amino acid analysis to determine the:
- Concentration of a purified protein when you know the known amino acid sequence.
- Total protein amount in a complex sample with unknown proteins.
- Accurate concentration on protein stock solution. You use it to calibrate other quantitative analyses, such as ELISA assays.
Amino acid analysis is the golden standard for accurate quantification of both proteins and peptides. The amino acid analysis process is quite simple: First a hydrolysis of an accurate amount of sample, and subsequently detection and quantification of individual amino acids. The analysis provides quantitative data for 18 out of 20 amino acids. However, degradation of tryptophan and cysteine occur during the hydrolysis.
Amino acid analysis:
- Choose between a single or Triplicate amino acid assay. We recommend analysis in triplicates, whenever you need a very accurate protein or peptide concentration determination.
- Comparison of results against your protein or peptide sequence.
- Optional: It is very useful to combine the amino acid assay with an Extinction Coefficient measurement at 280 nm.
We analyze your samples with the newest technology and equipment in only few days. You get the results in an easy-to-understand report, also understandable to the non-expert.