Instructions for use – GPMAW lite, the free protein calculation tool
GPMAW lite is a free bioinformatics/protein calculation tool. You can use it on any protein amino acid sequence. Alphalyse has developed the software in collaboration with Peter Hoejrup. Hoejrup also developed the full GPMAW program.
Click to start GPMAW lite now in a new window. Then return to this page for instructions on how to use the free tool.
3 simple steps
1. Enter the Sequence
You can use GPMAW lite to easily find the amino acid sequences for any known protein. The tool uses both the NCBI and UniProt databases to search for the sequence. First of all, you can write a sequence directly into the tool. Or if you wish, you can copy the sequence from other programs or documents. Just remember to always use the 1-letter code.
With the CLEAN function you remove all non-amino acid characters. This is for instance numbers, spaces, and special signs from the sequence. It also turns lowercase letters into uppercase.
Use the RESET function to clear all entries.
2. Choose your Analysis
Your can choose from three types of analysis:
Protein information. This function provides basic information about the protein:
• Basic molecular weight (mono-isotopic and average mass, non-reduced form).
• The theoretic calculated molar absorbance and extinction coefficient.
• Isoelectric point.
• Hydrophobicity index.
Amino Acid Composition. Use this analysis to show an amino acid composition table.
It contains numbers and % of individual amino acids.
In addition, any unusual amino acids in the sequence will be shown in the table.
Protease digest. This analysis shows you a list of peptides. They are formed by theoretical complete digestion using specific proteases.
The table contains info about:
• First of all the peptide sequence of your protein.
• Then follows MH+. (Mono-isotopic molecular weight of the protonated peptide in reduced form).
• Finally the pI and expected retention time in a HPLC run (reverse phase). This is included for each peptide.
All of the columns can be sorted. – You just click the header of the column.
3. Analyse the Results
Here you can enter the protein sequence as an accession number. Both the protein name and the accession number will then appear at the top of the Analysis Results page.
The COLORIZE function highlights specific amino acids. This also includes potential sites for N-glycosylation.
Finally, with the PRINT function (at the bottom of the page) you can print all of the Analysis Results. Afterwards, you may include the information in your lab books and project files. Please reference our site (www.alphalyse.com) if you publish a paper with data from our tool.