HCP Coverage Analysis

Do you know how well your ELISA covers the HCPs in your sample?

If you need to evaluate and qualify the antibodies of a generic or process specific HCP ELISA we can help you. We use an advanced MS-based coverage analysis method (ELISA-MS^™) which mimics the conditions of the ELISA, for more reliable and accurate results:

In this analysis, the host cell proteins in a mock lysate or an early process sample are immunocaptured using the ELISA antibodies and then identified by LC-MS/MS. As a result, you get not only the total sum of the residual HCPs, but also a list of the HCPs recognized by the ELISA antibodies.

How does ELISA-MS^™ analysis compare to 2D PAGE and Western blot?
ELISA-MS^™ is a proven method recognized as a better alternative to spot counting in 2D gel electrophoresis and Western blot, because it provides much more detailed and accurate information. An Immunocapture LC-MS analysis (ELISA-MS^™) will thus expand your knowledge of the HCP-ELISA’s specificity and applicability to your biopharmaceutical. It may also reduce the risk of project delays due to poorly documented HCP ELISA.

A standard ELISA-MS^™ HCP coverage analysis includes

• ELISA setup for optimal antibody-HCP binding.
• Immunocapture with ELISA polyclonal antibodies.
• Identification of both total HCPs and affinity purified HCPs by sensitive LC-MS/MS.
• Reporting of HCPs covered by antibodies and also a list of total HCPs in the mock lysate or early process sample.

Send us a description of your project and we will be glad to discuss it with you.