HCP Antibody Coverage Analysis

Do you know the HCPs covered by your ELISA?

If you need to evaluate the antibodies of a generic or process specific HCP ELISA we can help you through our MS-based coverage analysis method.

In this analysis, the host cell proteins in a mock lysate or an early process sample are immunocaptured using the ELISA antibodies and then identified by LC-MS/MS. As a result, you get a list of the HCPs recognized by the ELISA antibodies.

This is a better alternative to spot counting in 2D gel electrophoresis and western blot, because it provides much more detailed and accurate information. An Immunocapture LC-MS analysis will thus expand your knowledge of the HCP-ELISA’s specificity and efficiency. It may also reduce the risk of project delays due to poorly documented HCP ELISA.

A standard HCP coverage analysis includes

• ELISA setup for optimal antibody-HCP binding
• Immunocapture with ELISA polyclonal antibodies
• Identification of both total HCPs and affinity purified HCPs by sensitive LC-MS/MS
• Reporting of HCPs covered by antibodies and also a list of total HCPs in the mock lysate

Send us a description of your project and we will be glad to discuss it with you.