Latest news about Host Cell Protein

1 April 2020

Alphalyse A/S moves into new headquarters

It was quite nerve-wracking to watch the SCIEX TripleTOFs get moved into the new labs. Since the regular staircase was too steep and long, we had to get them in through the fire-escape on the 1st floor! You can watch a video of it here >> We are now getting adjusted to the new surroundings… Read whole story

5 March 2020

Research article published: Novel coverage analysis by ELISA-MS

In this newly published research article our scientists present a novel coverage method combining ELISA-based immunocapture with protein identification by liquid chromatography–tandem mass spectrometry (LC–MS/MS). Abstract Download article (open-access) >> Monitoring host cell proteins (HCPs) is one of the most important analytical requirements in production of recombinant biopharmaceuticals to ensure product purity and patient safety…. Read whole story

20 February 2020

Webinar: Design the optimal HCP Analysis strategy

Did you know that a ‘good’ HCP analysis strategy should … … be able to detect and document both broad-level HCPs and specific (critical) HCPs? In this 30 minute webinar Ejvind Mortz, COO, covers how to design a strategy for: • Detecting and documenting HCP impurities in process samples and final drug • Making a… Read whole story

12 September 2019

‘Pippi’ automates complex & variable protocols for protein analyses

Reproducible sample preparation is perhaps the most important part of protein analysis. See how we managed to fully automate complex sample preparation for high reproducibility – and create a good work environment for our team using an Eppendorf Automation robot. Read article on Eppendorf website >>

6 September 2019

Which HCP impurities is your ELISA assay missing?

Alphalyse now launches a new reproducible method for detecting which individual impurities are measured Join our webinar on Wednesday, 18 September 2019 to learn more: Attend if you are a manager or scientist in R&D or process development and concerned about the Host Cell Proteins (HCPs) in your biologic. ​​​​​​​ ​​​​​​​After participating, you.. → Possess… Read whole story

13 August 2019

Bioprocessing Summit: Time to rethink your HCP Coverage analysis?

Your HCP analysis should be both reactive to broader populations of HCPs and more specific to HCPs likely to be in the final product. However, with current methods, based on 2D PAGE and Western Blot, it is difficult to know if both of these conditions are met. If you’re in Boston today, you can experience… Read whole story

4 June 2019

Review of BEBPA HCP Conference 2019 – Key topics & take-home messages

BEBPA’s 7th annual Host Cell Protein Conference May 15-17th took place in Los Angeles, California. It once again attracted HCP experts from biopharma, service- and technology providers, and regulatory agencies. The organizing committee had again put together a great mix of talks, workshops and round-table discussions. Very importantly, there was also plenty of time for… Read whole story

2 May 2019

Alphalyse scientist successfully defends PhD thesis

CONGRATULATIONS to Katrine Pilely who Tuesday successfully defended her PhD thesis at the University of Copenhagen. Under supervision of Professor Peter Garred, Katrine Pilely studied how cholesterol crystals activate the immune system. This happens through complement-mediated mechanisms, and therefore contribute to the pathophysiology of atherosclerosis. Katrine further showed how to inhibit the upstream cholesterol crystal-induced… Read whole story

22 February 2019

Easy method for following specific HCPs through purification steps

See how Rikke Lund’s team helps with removal of host cell protein contaminants from recombinant protein with the use of mass spectrometry. In this short video Rikke gives examples of customer results. The host cell protein analysis provides the customer with both identity and quantity of individual HCPs. This makes it possible to compare and identify differences… Read whole story

31 January 2019

Document residual protein A in final drug substance

Standard analyses do not detect trace amounts of protein process additives, otherwise known as residual proteins. Examples include growth factors, Benzonase nuclease, Protein A, enzymes for site-specific PEGylation, etc. Instead you can now use a customized LC-MS assay, developed by Alphalyse. It will detect residual proteins of interest – in both your final drug substance… Read whole story

3 January 2019

2019 New Year Resolution: No more Western Blotting!

Yes, we are determined to wipe out Western Blotting as the standard HCP-ELISA coverage analysis. To meet customer demand, our HCP team is thus stepping up with yet another team-member; Katrine Pilely. Since new year, Katrine has taken part in the Alphalyse onboarding program and she is now ready to provide customers with useful information… Read whole story

6 December 2018

Poster: Detailed analysis of Host Cell Protein in process samples

Together with Sciex AB we have made a scientific poster on detailed analysis of the Host Cell Protein in process samples. The poster describes our fast LC-MS method that is based on SWATH technology. Since the method is generic, you can apply it to biopharmaceutical processes within a few weeks. The method can be used… Read whole story

2 October 2018

HCP presentation at “Biologics Characterization Made Easy” in CPH

On 2 October, Thomas Kofoed presented on “Identification and Absolute Quantification of Host Cell Proteins using SWATH Mass Spectrometry.” SCIEX and Phenomenex hosted this event on the recent developments in analytical technologies for biopharmaceutical characterization using Mass Spectrometry and Capillary Electrophoresis. Experts in the field presented the latest innovations in: Routine and advanced workflows for… Read whole story

19 June 2018

Training on the Sciex X500B QTOF

Kerstin Poll from Sciex is teaching us how to get the most out of our new X500B QTOF. Soon we will be able to help you with new quantitative peptide maps by MAM. We will also be able to find variants of protein pharmaceuticals, and make even better analysis of product related impurities.

14 June 2018

Developing new analysis in collaboration with customers and suppliers

Not satisfied with your current protein analysis method?  Let’s improve it! In this new article you get an example of how we invent new analysis and improve on existing methods in collaboration with our customers and suppliers – leading to cutting-edge LC-MS technology that specifically addresses your challenges. ► “It’s a perfect example of… Read whole story

23 May 2018

BEBPA 2018 – Key topics and take-home messages

The BEBPA HCP meeting 2018 was this time held in Europe – more precisely in beautiful Dubrovnik, Croatia. The conference was again very well planned with a great mix of talks from industry, academia and regulatory authorizations. The BEBPA HCP meeting is the perfect place to get the full picture about current best practice for HCP… Read whole story

11 April 2018

Ejvind Mortz presents at ESAC by AMCHAM

Today you can meet Ejvind Mortz at the ESAC meeting in Copenhagen. Come and learn how Host Cell Protein analysis by SWATH LC-MS provides: Identification & quantification of each HCP. – Which makes it very easy to compare individual HCPs for both different process samples & final batches. Direct application of the analysis to your samples…. Read whole story

22 March 2018

Got plans for Easter?

So why not spent 30 minutes to read this new article on HCP analysis by SWATH® LC-MS? “Evaluation of spectral libraries and sample preparation for DIA-LC-MS analysis of host cell proteins. A case study of a bacterially expressed recombinant biopharmaceutical protein”. Use this link to get 50 days’ free access to the full article. No… Read whole story

1 February 2018

Cobot for automated protein sample preparation of biologics

Over the past few weeks, Hannah and Michael (Eppendorf) have helped us set up our first automated protein sample preparation protocol for biopharmaceuticals on “Pippi” (epMotion pipetting system). Pippi is our new Cobot (Collaborative Robot) which will help us optimize the work in our digest lab. The goal is to automate standard procedures to further… Read whole story