Peptide mapping services

Do you want to verify the sequence of your protein or antibody?

Peptide mapping using mass spectrometry is one of the most valuable methods for verifying the amino acid sequence of proteins. Therefore, the analysis is an integral part of the protein characterization needed for biologics, as stated in ICH Q6B guidelines.

You can apply peptide mapping to confirm protein identity for lot release and to analyze product-related impurities with stability and forced degradation studies.

Careful selection of proteases for the digest can optimize the sequence coverage. Using our peptide mapping services, you will also get detailed information about post-translational modifications – including deamidations and oxidations.

Service 1: Peptide Mapping

• Digestion with trypsin cleaving after Arg and Lys residues.
• Analysis of the resulting peptide mixture by single injection UV-LC-MS/MS
• Peptide map of observed peptides against the theoretical sequence
• Results within two weeks

Service 2: Quantitative Peptide Mapping

• Digestion with suitable proteases based on bioinformatics analysis of the protein sequence
• Analysis of the resulting peptide mixture by triplicate injection UV-LC-MS/MS
• Peptide map of observed peptides against the theoretical sequence
• Quantification of post-translational modifications
• Results within four weeks

Common modifications we look for include Asn/Gln deamidation, N-term pyroglu, C-terminal Lys truncation, and M/W oxidation.

Service add-ons:

• Multiple protease digestions, e.g., Lys-C, Glu-C, chymotrypsin, Asp-N, Arg-C
• Custom choice of LC gradient and column
• Search for custom/specific post-translational modifications