Database Searching & Protein Identification

5 articles

Speedy and detailed protein ID of SDS gel bands

Question: Can I identify hundreds of gel bands in a few days? The biopharmaceutical company that I work for, consistently uses SDS...

Jun 9. 2020 | Thomas Kofoed |
Protein molecular weight calculator

GPMAW lite – Protein Sequence Calculator (and much more)

When working with proteins you often need to make simple bioinformatics calculations on a specific protein sequence - and for...

Apr 10. 2017 | Thomas Kofoed |
Mass spectrometry analysis of protein

Common protein contaminants in mass spectrometric protein ID

Questions: Why was Keratin identified in my 1D gel band? Is the keratin determined by the protein identification service in my...

Jun 3. 2016 | Ejvind Mørtz |
Eppendorf tubes with protein

Mass spectrometric identification of proteins from Western blots

Question: Is there a protocol for preparing PVDF membrane for mass spectrometry? We have an unknown protein recognized in Western blot by...

May 2. 2016 | Ejvind Mørtz |

What does the Mascot Score mean?

Question: What does the Mascot Score mean? I just got my protein identification report. What does the Mascot Score mean? Answer: The Protein Identification...

Oct 9. 2015 | Ejvind Mørtz |
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