Is there a simple way to perform label-free quantification of Host Cell Proteins?
Question: I find it difficult to get an accurate label-free quantification of Host Cell Proteins with LC-MS. It also seems that...
Quickly compare HCP in different process steps, without setting up an ELISA
Question: I have spent days trying to set up a useful ELISA assay for detecting Host Cell Proteins (HCPs) in my process samples. I'm...
What is SWATH and why is it an advantage for Host Cell Protein analysis?
Host Cell Proteins (HCPs) in protein biopharmaceuticals can be analyzed by mass spectrometry - for both identification and quantification of...
Identification of small host cell proteins (low Mw HCPs)
Question: Could you suggest an analysis method to detect low Mw HCPs (small HCPs) below 20 kDa? I work on a drug...
3 steps to identify and quantify HCP in a drug substance homolog from a host cell
Question: My HCP antibodies show a reaction towards a drug substance band in my Western blot. Is it possible that it is...
Mass Spectrometry for Host Cell Protein monitoring and control in pharmaceutical process development
Mass spectrometry based Host Cell Protein identification and quantification Here the Mass Spec Host Cell Protein (HCP) assay is applied for...