7 articles
1000 proteins quantified in e. coli mock lysate - HCP

Is there a simple way to perform label-free quantification of Host Cell Proteins?

Question: I find it difficult to get an accurate label-free quantification of Host Cell Proteins with LC-MS. It also seems that...

Jun 25. 2018 | Ejvind Mørtz | 0

What are “Jackpot HCPs” and why do I need a “lucky goat” to get a precise result in my HCP ELISA?

Question: I tested two different HCP ELISA kits on my biopharmaceutical. Why do they give different results and which one is...

May 28. 2018 | Anette Draborg | 0

Quickly compare HCP in different process steps, without setting up an ELISA

Question: I have spent days trying to set up a useful ELISA assay for detecting Host Cell Proteins (HCPs) in my process samples. I'm...

Apr 10. 2018 | Ejvind Mørtz | 0
What is SWATH?

What is SWATH and why is it an advantage for Host Cell Protein analysis?

Host Cell Proteins (HCPs) in protein biopharmaceuticals can be analyzed by mass spectrometry - for both identification and quantification of...

Jun 22. 2017 | Thomas Kofoed | 0
Analysis method for Mw HCPs (small HCPs) below 20 kDa

Identification of small host cell proteins (low Mw HCPs)

Question: Could you suggest an analysis method to detect low Mw HCPs (small HCPs) below 20 kDa? I work on a drug...

Oct 11. 2016 | Ejvind Mørtz | 0
Protein scientist at Alphalyse

3 steps to identify and quantify HCP in a drug substance homolog from a host cell

Question: My HCP antibodies show a reaction towards a drug substance band in my Western blot. Is it possible that it is...

Sep 5. 2016 | Rikke Raaen Lund | 0

Mass Spectrometry for Host Cell Protein monitoring and control in pharmaceutical process development

Mass spectrometry based Host Cell Protein identification and quantification Here the Mass Spec Host Cell Protein (HCP) assay is applied for...

Oct 2. 2009 | Ejvind Mørtz | 2
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