Analysis of Host Cell Protein

9 articles
Adenovira

Why measure process-related impurities in gene therapies with LC-MS?

Question: I work in a biopharmaceutical company developing a gene therapy product based on an adenovirus vector. Recently, I have become...

Dec 2. 2020 | Ejvind Mørtz |

How to document removal of a process related impurity

Question: My company develops and produces a biologic. Recently, regulatory authorities asked us to document how efficiently downstream purification reduces the...

Sep 25. 2019 | Ejvind Mørtz |
ELISA coverage

Can I use commercial HCP ELISA kits in clinical trial documentation?

Question: My company uses a commercial HCP ELISA kit for host cell protein analysis of clinical phase 1 material. We hear...

Jul 10. 2019 | Ejvind Mørtz |

What are Host Cell Proteins (HCPs)?

Question: I recently met the term Host Cell Proteins (HCPs) in relation to process development and production of biopharmaceuticals. What are...

Jan 10. 2019 | Ejvind Mørtz |
1000 proteins quantified in e. coli mock lysate - HCP

Simple way to perform label-free quantification of Host Cell Proteins

Question:   I find it difficult to get an accurate label-free quantification of Host Cell Proteins with traditional multiple reaction monitoring using...

Jun 25. 2018 | Ejvind Mørtz |

How do ‘Jackpot HCPs’ and a ‘Lucky Goat’ influence my ELISA results?

Question: I tested two different HCP ELISA kits on my biopharmaceutical. Why do they give different results and which one is...

May 28. 2018 | Anette Draborg |
What is SWATH?

What is SWATH mass spectrometry and its advantages in HCP analysis?

Host Cell Proteins (HCPs) in protein biopharmaceuticals can be analyzed by the mass spectrometry SWATH analysis. It provides both identification...

Jun 22. 2017 | Thomas Kofoed |
Analysis method for Mw HCPs (small HCPs) below 20 kDa

Identification of small host cell proteins (small Mw HCPs)

Question: I work on a drug substance protein with a mass of 15 kDa. I would expect small host cell protein...

Oct 11. 2016 | Ejvind Mørtz |

Host Cell Protein monitoring in process development – using mass spec

Mass spectrometry based Host Cell Protein identification and quantification The Mass Spec Host Cell Protein (HCP) assay is applied for monitoring...

Oct 2. 2009 | Ejvind Mørtz |
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