Multiple Reaction Monitoring
- Quantify specific proteins in a complex mixture
- Identify up- or down-regulation of specific proteins
- Save time for development of ELISA assay
We offer customized solutions, contact us to discuss your project.
Send us a description of your project and we shall be happy to present you with a solution.
We encourage you to call us or schedule a meeting to discuss your project in detail.
Set up meetingProtein characterization for optimization of manufacturing processes.
See moreOptimized HPLC analysis of peptides for clinical trials and stable GMP production
See moreHigh-throughput characterization analysis of antibodies for fast quality assessment
See moreWe would like to help you as much as possible with your project and therefore provide several kinds of customer support:
The absolute quantification of specific protein in complex matrices are based on the selection of specific peptides as signature peptides. We use it for assay development based on Multiple-Reaction-Monitoring (MRM). A project is typically divided into three parts:
The purpose of the feasibility study is to investigate the possibility to develop an assay that can be used for the quantification of the specific protein in the matrix. The feasibility study includes:
After we agreed to start development of an assay we usually follow these steps:
When the final assay is ready, we measure real samples and prepare the report. We also offer to go over the results at a telephone conference so you can get answer to possible questions regarding the data.
Protein quantification by mass spectrometry requires a protein in sufficient amounts to obtain good data. It is thus important that samples are prepared in a clean laboratory to avoid contamination with human keratin. To develop MRM quantification assays requires a protein in pure form and a matrix sample without the protein similar to the matrix of the real samples to be analyzed.
Protein samples can be submitted in liquid or lyophilized.