Multiple Reaction Monitoring
- Quantify specific proteins in a complex mixture
- Identify up- or down-regulation of specific proteins
- Save time for development of ELISA assay
We offer customized solutions, contact us to discuss your project.
Send us a description of your project and we shall be happy to present you with a solution.
We encourage you to call us or schedule a meeting to discuss your project in detail.
Set up meetingProtein characterization for optimization of manufacturing processes.
Explore customer caseOptimized HPLC analysis of peptides for clinical trials and stable GMP production
Explore customer caseHigh-throughput characterization analysis of antibodies for fast quality assessment
Explore customer caseWe would like to help you as much as possible with your project and therefore provide several kinds of customer support:
The absolute amount of a specific protein in complex matrices is based on selecting specific peptides as signature peptides. We use it for assay development based on Multiple-Reaction-Monitoring (MRM). A typical project consists of three parts:
The purpose of the feasibility study is to investigate the possibility of developing an assay that can be used to quantify the specific protein in the matrix. The feasibility study includes:
After we agree to start the development of an assay, we usually follow these steps:
We measure actual samples and prepare the report when the final assay is ready. We also offer to go over the results at a telephone conference so you can get answers to possible questions regarding the data.
Protein measurement by mass spectrometry requires a protein in sufficient amounts to obtain good data. Developing MRM quantification assays requires a protein in pure form and a matrix sample without the protein similar to the matrix of the actual samples to be analyzed. Thus, please prepare the samples in a clean laboratory to avoid contamination with human keratin.
You can submit protein samples in liquid or lyophilized.