We can list up to 40 amino acids of your drug substance by using N-terminal Edman sequencing (Edman degradation). Our experts perform the analysis, using the best available technology.
N-terminal Edman sequencing is a cyclic procedure. We cleave off the amino acid residues one at a time. Then we identify them by chromatography. However, the analysis will be unsuccessful for samples containing multiple N-terminals and for N-terminally blocked proteins.
PROTEIN – sequencing service includes:
- Optional 1D SDS PAGE or HPLC isolation of the protein.
- Sequencing by Edman degradation.
- Determination of up to 40 amino acids.
- Comparison of data to expected drug substance sequence.
ANTIBODY – sequencing service includes:
- 1D SDS PAGE separation of the HC & LC.
- Blotting onto PVDF membrane.
- Staining and also cutting of the HC & LC bands.
- N-terminal Edman sequencing – up to 40 amino acids for each chain.
- Optional: De-blocking of pyroglutamic acid.
Data is delivered in an easy-to-understand report.
Download application note (pdf – 1 MB). Learn more about the many applications of Edman degradation.